Docetaxel is a semi-synthetic anticancer drug processing from the European yew needles extract. Its structure is similar to paclitaxel. Sources of docetaxel are more accessible compared with paclitaxel. The killing effect of certain tumor is 2 times than that of paclitaxel. It is still valid to some resistant cells of over-expression of P-glycoprotein. But the role of docetaxel in gastric cancer cells can not be fully understood.

Docetaxel has improved significantly MKN45 cells and MKN45 cells to prevent the concentration of inhibitor. With different concentrations of docetaxel in gastric cancer cells, cells in agarose gel electrophoresis as Tuner mice, each treatment group showed that cell apoptosis was significantly higher in number, gel electrophoresis, DNA, and a full card depending on ability level, as shown is.

Docetaxel-treated cells, cells stain in real-time PCR quantity and Western analysis were collected at different times of use. The results are compared with the control group were treated with significantly lower mRNA and protein levels. Docetaxel is used in clinical treatment of gastric cancer, and gastric cancer cells and the approach is clear. Scientists docetaxel MKN45 cells, apoptosis and survival using gene therap

Apoptosis, cell and frequency of tumor formation plays a key role in the regulation. Gastric cancer cells with different concentrations of docetaxel treatment, the cells collected at different times in the tunnel, and agarose gel electrophoresis method, which showed a big problem to find time and concentration dependent apoptosis. At the same time, malignant cells, stomach and prevents the concentration of the drug. Results for stomach cancer, recommended the establishment can prevent cell division and apoptosis associated.

This is a classic way apoptosis, mitochondrial and track death receptor mechanisms. Apoptosis signaling by inhibiting apoptosis inhibitor of apoptosis protein family. Factors important to live in the IAP family. Cell and tissue research, survival is almost mature, differentiation and expression, and also in embryonic tissues and lungs full breast tissue from different tumor tissues, high expression is case sensitive. Survival and resistance factors and no small cell lung cancer, stomach cancer, neurosurgery Blas Tomas, to reduce tumors in patients with increased expression of their survival. High expression of gastric cancer tissues to survive in the surrounding tissue expression, but high expression reduced apoptotic index and a positive effect on tumor invasion in gastric cancer

After docetaxel’s treatment on gastric cancer cells, the scientists test the PCR and Western blot analysis of mRNA and protein levels. Pharmaceutical raw materials suppliers find that the in treatment groups, the survival protein and mRNA decrease. In addition to the decreasing of survivin expression, the level of tumor cell apoptosis has a corresponding rise. According to the statistical analysis, they show a positive correlation. Survivin plays an important role in docetaxel-induced apoptosis of gastric cancer. The study declares that in vitro, docetaxel can significantly inhibit the proliferation of gastric cancer cells. Its mechanism may be related to induction of apoptosis and downregulation of surviving gene expression.Source:http://www.cospcn.com